Kenta Iitani, Toshiyuki Sato, Xin Wang, Koji Toma, Takahiro Arakawa, Kohji Mitsubayashi: “Sniffer-camera” using enzyme reaction for visualization of transpired ethanol from palm skin. In: Journal of Advanced Science, 26 (3+4), pp. 20–22, 2014, (Letter).

Abstract

An imaging system for gaseous ethanol transpired from human palm skin, based on an enzymatic reaction, was assembled and validated. This system uses a highly sensitive camera that measures gaseous ethanol concentrations as intensities of chemiluminescence from luminol’s reaction induced by alcohol oxidase (AOD) and a luminol-hydrogen peroxide-horseradish peroxidase (HRP) system. Conversion of gaseous ethanol concentrations and distributions proceed on an enzyme-immobilized mesh substrate with luminol solution in a dark box. In order to visualize ethanol transpired from human palm skin, we improved the chemiluminescence sensitivity of the imaging system with a mixture of a high-purity luminol solution of luminol sodium salt HG solution and an enhancer of eosin Y solution. The detection limit of the ethanol concentration was 4.9 ppm. This highly sensitive imaging allows successful visualization of ethanol transpired from palm skin.

BibTeX (Download)

@article{Iitani2014,
title = {“Sniffer-camera” using enzyme reaction for visualization of transpired ethanol from palm skin},
author = {Kenta Iitani and Toshiyuki Sato and Xin Wang and Koji Toma and Takahiro Arakawa and Kohji Mitsubayashi},
url = {https://www.jstage.jst.go.jp/article/jsas/26/3+4/26_150225699/_article/-char/en},
doi = {10.2978/jsas.26.20},
year  = {2014},
date = {2014-05-20},
journal = {Journal of Advanced Science},
volume = {26},
number = {3+4},
pages = {20–22},
abstract = {An imaging system for gaseous ethanol transpired from human palm skin, based on an enzymatic reaction, was assembled and validated. This system uses a highly sensitive camera that measures gaseous ethanol concentrations as intensities of chemiluminescence from luminol’s reaction induced by alcohol oxidase (AOD) and a luminol-hydrogen peroxide-horseradish peroxidase (HRP) system. Conversion of gaseous ethanol concentrations and distributions proceed on an enzyme-immobilized mesh substrate with luminol solution in a dark box. In order to visualize ethanol transpired from human palm skin, we improved the chemiluminescence sensitivity of the imaging system with a mixture of a high-purity luminol solution of luminol sodium salt HG solution and an enhancer of eosin Y solution. The detection limit of the ethanol concentration was 4.9 ppm. This highly sensitive imaging allows successful visualization of ethanol transpired from palm skin.
},
note = {Letter},
keywords = {alcohol oxidase, chemiluminescence, Ethanol, Skin gas},
pubstate = {published},
tppubtype = {article}
}